Download the .jar or .class file for Colour Deconvolution 2 from the official Birmingham Blogs site.
Restart ImageJ or use to make the command appear. Key Features and Functions color deconvolution imagej download
Place the downloaded file into the folder on your computer. Download the
To perform stain separation in histological imaging, the Colour Deconvolution plugin is the standard tool for ImageJ and Fiji. This plugin implements the Ruifrok and Johnston method to unmix up to three different colors from a single RGB image. To perform stain separation in histological imaging, the
You likely already have it. The plugin is bundled with Fiji. To find it, press "L" to open the Command Finder and type "decon". Alternatively, navigate to Image > Color > Colour Deconvolution . For ImageJ1 Users:
The plugin is primarily used for brightfield microscopy where light-absorbing dyes (like H&E or DAB) mix subtractively. Colour Deconvolution - Fiji Menu path. Image › Color › Colour Deconvolution. Fiji: ImageJ, with "Batteries Included" Colour Deconvolution - ImageJ - Fiji
Installation depends on whether you are using the standard ImageJ1 or the bundled Fiji distribution: