Follow the prompts to select an installation directory (e.g., C:\Program Files\NCBI\blast-2.17.0+ ).
Look for files ending in .exe (e.g., ncbi-blast-2.17.0+-win64.exe ). macOS: Look for the .dmg installer or the .tar.gz archive. download blast command line
To run BLAST from any command prompt, add the bin folder of your installation to your System Environment Variables (PATH). Follow the prompts to select an installation directory (e
Download the .tar.gz archive appropriate for your architecture (e.g., x64-linux ). 2. Installation Guide by OS Run the downloaded .exe installer. To run BLAST from any command prompt, add
The official source for the latest BLAST+ binaries is the NCBI FTP server . You should download the version specific to your operating system:
How to Download and Install BLAST Command Line (BLAST+) Downloading the applications—collectively known as BLAST+ —allows you to run sequence alignments locally on your own machine. This is essential for handling large-scale data, integrating BLAST into automated workflows, or using custom private databases. 1. Download the BLAST+ Executables
Verify the installation by opening the and typing blastn -version . BLAST+ executables — BLASTHelp documentation - NIH