A substrate (e.g., TMB or PNPP) is added. The enzyme reacts with this substrate to produce a color, which is measured using a spectrophotometer at specific wavelengths, commonly 450 nm. The 4 Major Types of ELISA ELISA: What It Is, Purpose, Procedure & Results
The , also known as enzyme immunoassay (EIA), is a gold-standard laboratory technique used to detect and quantify substances such as peptides, proteins, antibodies, and hormones. It relies on highly specific interactions between an antigen and an antibody, where a reporter enzyme creates a measurable color change to indicate the presence of the target analyte. Core Principles and Procedure elisa test pdf download
The sample is added, followed by incubation with enzyme-linked antibodies that bind to the target molecule. A substrate (e