Hibit Best ◉
HiBiT is an engineered 11-amino-acid peptide tag () with a very high affinity for its counterpart, a larger 18 kDa protein called LgBiT (Large BiT). When these two components meet, they spontaneously dimerise to form a functional luciferase enzyme.
By adding LgBiT to the culture medium, researchers can monitor the secretion or translation of proteins in real-time. 2. Virology and Vaccine Development HiBiT is an engineered 11-amino-acid peptide tag ()
The versatility of the HiBiT system allows it to be used in various experimental setups, from high-throughput drug screening to real-time protein monitoring. 1. Protein Quantification and Turnover HiBiT is an engineered 11-amino-acid peptide tag ()