Oligoanalyzer Download - |work|

Using the most accurate "Nearest Neighbor" thermodynamic parameters. GC Content: Crucial for stability and annealing efficiency.

more negative than -9 kcal/mol is likely to cause issues in the lab. oligoanalyzer download

This tool predicts the stability of internal loops. A primer that folds on itself won't bind to your template. Look for the Delta G ( ΔGcap delta cap G oligoanalyzer download