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Disruption of the OsDUR3 gene via T-DNA insertion has been shown to reduce rice growth on urea and decrease urea uptake, highlighting its essential role. 2. Internal Urea Retranslocation

OsDUR3 is a high-affinity urea transporter in rice ( Oryza sativa ) that belongs to the , which is well-known for active urea uptake. It is a plasma membrane-localized, integral membrane protein consisting of roughly 721 amino acid residues and 15 predicted transmembrane domains.

) are the primary forms of nitrogen absorbed by plants, urea is widely applied as a key chemical N fertilizer in modern agriculture due to its high nitrogen content. However, the efficient utilization of urea by crops depends heavily on specialized transport mechanisms. Among these, (Oryza sativa DUR3), a high-affinity urea transporter, has emerged as a critical player in efficient urea acquisition and utilization in rice, particularly under nitrogen-deficient conditions. Disruption of the OsDUR3 gene via T-DNA insertion

This article explores the molecular mechanism of OsDUR3, its role in rice production, and how it contributes to enhancing Nitrogen Use Efficiency (NUE) in agricultural systems. What is OsDUR3?

OsDUR3 expression is significantly upregulated in rice roots under nitrogen-deficiency and during urea-resupply after nitrogen-starvation. It is a plasma membrane-localized, integral membrane protein

-symporter) high-affinity transporter. This allows rice to absorb low-concentration urea from the soil, especially in Nitrogen-limited environments where urease has already hydrolyzed much of the urea into other forms. Molecular Mechanism and Structure

OsDUR3 plays a crucial role in two main areas: from the soil and internal N-recycling within the plant. 1. High-Affinity Root Uptake Among these, (Oryza sativa DUR3), a high-affinity urea

OsDUR3 contributes significantly to rice production under field conditions, making it a critical focus for increasing nitrogen use efficiency. Future Prospects: Enhancing Nitrogen Use Efficiency